Metabolism of linoleic and alpha linolenic acids in cultured cardiomyocytes: effect of different n-6 and n-3 fatty acid supplementation.
Metabolism of linoleic and alpha linolenic acids in cultured cardiomyocytes: effect of different n-6 and n-3 fatty acid supplementation.
Year: 1996
Authors: A Bordoni, J A Lopez-Jimenez, C Spano, P L Biagi, D F Horrobin, S Hrelia.
Publication Name: Mol. Cell. Biochem.
Publication Details: Volume 157; Numbers 1&2; Page 217.
Abstract:
Previous research by this group indicated that heart tissue is able to desaturate LA. In this study alterations in PUFA metabolism induced by the administration of n-3 PUFAs on heart myocytes were investigated. The ability of cultured cardiomyocytes to metabolize both LA and ALA in vivo in the presence or absence of GLA, EPA and DHA, either alone or combined, was assessed in cultured cardiomyocytes grown in different media. Cardiomyocytes were able to desaturate and elongate LA. GLA supplementation had no effect on LA conversion to more unsaturated fatty acids, whereas the addition of EPA and DHA, either alone or in combination, significantly decreased the formation of longer chain PUFAs from LA. GLA as well as EPA supplementation decreased the conversion of ALA in cardiomyocytes. When using a combination of GLA, EPA and DHA, GLA appeared to counterbalance the inhibitory effect of EPA and DHA on LA desaturation and elongation. GLA, EPA and DHA were all incorporated into cardiomyocyte lipids. GLA supplementation, either alone or in combination with n-3 PUFAs, positively affected cell proliferation. The authors indicated that since important risk factors for coronary heart disease may be associated with reduced 6- desaturation of the parent EFAs, administration of n-6 or n-3 EFA metabolites alone could cause undesirable effects. These EFAs appear to have different and synergistic roles and thus combined treatment with both families of EFAs appears to be preferable to the supplementation of either the n?6 or n-3 EFAs alone.
