Phytoestrogens and their metobolites inhibit the sensitivity of the bovine corpus luteum to luteotropic factors.

The aim of this study was to examine whether active metabolites of phytoestrogens (equol and para-ethyl-phenol) inhibit sensitivity of bovine corpus luteum (CL) to lutenizing hormone (LH) and to auto/paracrine luteotropic factors (prostaglandin E2-PGE2 and prostaglandin F2a-PGF2a), and whether they influence pulsatile progesterone (P4) secretion by the bovine CL. In in vivo experiments, high levels of equol and para-ethyl-phenol were found in plasma and in the CL tissue of heifers and cows fed a soy bean diet (2.5 kg/animal/day), along with lower concentrations of P4 (P<0.05). Both Prostaglandins (PG) and LH strongly simulated P4 secretion in cultured pieces of CL that were collected from cows fed a standard diet (P<0.01). There was ne effect of PGs and LH on P4 stimulation in CLs obtained from cows fed a diet rich in soy bean. Finally, we examined whether active metabolites of phytoestrogens participated in regulation of pulsatile P4 secretion and LH-stimulated P4 secretion in vitro using a microdialysis system. Equol and para-ethyl-phenol had no effect on basicand pulsatile P4 secretion in CLs during 240 min of perfusion when compared to the control (P<0.05).  However, they inhibited LH-stimulated P4 secretion (P<0.05). Phytoestrogens and their metabolites may disrupt CL function by inhibiting PG- and LH-stimulated P4 secretion. Author's Abstract.